Course Title : Environmental Organic Micropollutants Analysis Lab.

Code 10F468
Course Year Master and Doctor Course
Term Intensive course (25,27,28th Sep.)
Class day & Period 9:00 am- 6:00 pm
Location Seminer Room, Research Center for Environmental Quality Management
Credits 2
Restriction around 10 students
Lecture Form(s) Intensive Lecture
Language Japanese
Instructor Shimizu, Yoshihisa,Matsuda, Tomonari,

Course Description

There is increasing concern about proper risk evaluation and management of hazardous chemicals such as dioxins and endocrine disruptors. To manage this problem, it is necessary to understand analytical methods and toxicity of those hazardous chemicals. In this class, lectures and experiments will be carried out about chromatography, bioassays and mass spectrometry.


It is required to attend all 3 days for lectures and experiments. Attendance and reports are considered for grading.

Course Goals

Understand about principle and practical techniques of chromatography. Understand about principle of several bioassays.

Course Topics

Theme Class number of times Description
HPLC -How to separate it- 3 Learn about principle and practice of HPLC separation. How do you choose columns, solvents and detectors? How to improve peak separation?
Fractionation and Purification by using HPLC 3 Learn about practical techniques of fractionation and purification using HPLC.
LC/MS/MS 5 Learn about principle and practice of LC/MS/MS analysis. Understand about 3 different scan modes, full scan, daughter scan and MRM. How to make an analytical method in a refined way for substances of your interest.
Bioassays 4 Lecture about several bioassays which are used for evaluation of environmental toxicity, and discuss about how to identify toxic compounds in environment by using HPLC in combination with bioassays.


Handouts are distributed.


Daniel C. Harris: Quantitative Chemical Analysis ISBN-13: 978-1-4292-3989-9


Independent Study Outside of Class

We hope active participation of students. It is welcome that patticipants additionally try to analyze the sample their own interest.

Web Sites

Additional Information

This intensive course is useful especially for students who usually use or intend to use HPLC and LC/MS/MS for their research.